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KMID : 0606920060140040246
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Biomolecules & Therapeutics
2006 Volume.14 No. 4 p.246 ~ p.252
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Genetic Toxicity Teat of 1,2-Dibromoethane by Ames, Micronucleus,Comet Assays and Microarray Analysis
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Kim Ki-Y.
Kim Ji-H.
Kwon kyoung-J.
Go Seo-Y.
Min Kyung-N.
Lee Woo-S.
Park Sue-N.
Sheen Yhun-Y.
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Abstract
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1,2-Dibromoethane(DBE) has been widely used as a soil fumigant, an additive to leaded gasoline and an industrial solvent. In this study, we have carried out in vitro genetic toxicity test of 1,2-dibromoethane and microarray analysis of differentially expressed genes in response to 1,2-dibromoethane. 1,2-Dibromoethane showed mutations in base substitution strain TA1535 both with and without exogenous metabolic activation. 1,2- Dibromoethane showed mutations in frame shift TA98 both with and without exogenous metabolic activation. 1,2-Dibromoethane showed DNA damage based on single cell gel/comet assay in L1578Y cells both with and without exogenous metabolic activation. 1,2-Dibromoehane imcreased micronuclei in CHO cells both with and without exogenous metabolic activation. Microarray analysis of gene expression profiles in L5178Y cells in respones to 1,2-dibromoethane selected differentially expressed 241 genes that would be candidate biomarkers of genetic toxic action of 1,2-dibromoethane.
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KEYWORD
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1,2-Dibromoethane, Ames test, COMET, MN assay, Microarray, S9 fraction
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